Research Area D

2013

  Cyclin-dependent kinase 9 links RNA Polymerase II transcription to processing of ribosomal RNA
  Structures of Drosophila Cryptochrome and Mouse Cryptochrome1 Provide Insight into Circadian Function
  Structural basis of histone H2A–H2B recognition by the essential chaperone FACT
  Histone H3 Glutathionylation in Proliferating Mammalian Cells Destabilizes Nucleosomal Structure
  The neural crest transcription factor Brn3a is expressed in melanoma and required for cell cycle progression and survival
  Functional ribosome biogenesis is a prerequisite for p53 destabilisation: impact of chemotherapy on nucleolar functions and RNA metabolism.
  Suv4-20h2 mediates chromatin compaction and is important for cohesin recruitment to heterochromatin
  In vivo live imaging of RNA polymerase II transcription factories in primary cells
  TLR Activation Excludes Circulating Naive CD8+ T Cells from Gut-Associated Lymphoid Organs in Mice
  H3K56me3 Is a Novel, Conserved Heterochromatic Mark That Largely but Not Completely Overlaps with H3K9me3 in Both Regulation and Localization
  Dynamic Readers for 5-(Hydroxy)Methylcytosine and Its Oxidized Derivatives
  Histone H4 Lysine 20 methylation: key player in epigenetic regulation of genomic integrity
  Circadian acetylome reveals regulation of mitochondrial metabolic pathways
  Therapeutic Efficacy of Bifunctional siRNA Combining TGF-β1 Silencing with RIG-I Activation in Pancreatic Cancer

2012

  CTD serine-2 plays a critical role in splicing and termination factor recruitment to RNA polymerase II in vivo
  Global DNA Hypomethylation Prevents Consolidation of Differentiation Programs and Allows Reversion to the Embryonic Stem Cell State
  Controlling The Mobility Of Oligonucleotides In The Nanochannels Of Mesoporous Silica
  Towards a structural understanding of PARP1 activation and related signalling ADP-ribosyl-transferases
  Translation Elongation Factor EF-P Alleviates Ribosome Stalling at Polyproline Stretches
  Identification of a Target Gene and Activating Stimulus for the YpdA/YpdB Histidine Kinase/Response Regulator System in Escherichia coli
  The ATPase domain of ISWI is an autonomous nucleosome remodeling machine
  Direct and Dynamic Detection of HIV-1 in Living Cell
  Impact of Histone H4 Lysine 20 Methylation on 53BP1 Responses to Chromosomal Double Strand Breaks
  Autoinducers Act as Biological Timers in Vibrio harveyi
  MSL2 Combines Sensor and Effector Functions in Homeostatic Control of the Drosophila Dosage Compensation Machinery
  Protective and Aggravating Effects of Nlrp3 Inflammasome Activation in IBD Models: Influence of Genetic and Environmental Factors
  Histone H2A variants in nucleosomes and chromatin: more or less stable?
  Step-Wise Assembly, Maturation and Dynamic Behavior of the Human CENP-P/O/R/Q/U Kinetochore Sub- Complex
  Deactivation of the E. coli pH Stress Sensor CadC by Cadaverine
  Global analysis of genome, transcriptome and proteome reveals the response to aneuploidy in human cells
  Tyrosine-1 and threonine-4 phosphorylation marks complete the RNA polymerase II CTD phospho-code
  Dynamic phosphorylation patterns of RNA polymerase II CTD during transcription
  Myb-binding Protein 1a (Mybbp1a) Regulates Levels and Processing of Pre-ribosomal RNA
  Nucleosome Remodeler SNF2L Suppresses Cell Proliferation and Migration and Attenuates Wnt Signaling
  CTD Tyrosine Phosphorylation Impairs Termination Factor Recruitment to RNA Polymerase II
  A unified phylogeny-based nomenclature for histone variants
  First insights into the unexplored two-component system YehU/YehT in Escherichia coli
  Phosphorylation by cyclin-dependent kinase-9 controls ubiquitin-conjugating enzyme-2A function
  Versatile Toolbox for High Throughput Biochemical and Functional Studies with Fluorescent Fusion Proteins
  EMBO welcomes 3 CIPSM scientists as new members
  Cascaded Photoinduced Drug Delivery to Cells from Multifunctional Core–Shell Mesoporous Silica
  Threonine-4 of mammalian RNA polymerase II CTD is targeted by Polo-like kinase 3 and required for transcriptional elongation
  Ultra short and progressive 4sU-tagging reveals key characteristics of RNA processing at nucleotide resolution
  H2A.Z.2.2 is an alternatively spliced histone H2A.Z variant that causes severe nucleosome destabilization
  Targeted transcriptional activation of silent oct4 pluripotency gene by combining designer TALEs and inhibition of epigenetic modifiers
  ATRX-mediated chromatin association of histone variant macroH2A1 regulates a-globin expression
  Structure, function and dynamics of nuclear subcompartments
  Cytosolic RIG-I–like helicases act as negative regulators of sterile inflammation in the CNS
  Polycomb Associates Genome-wide with a Specific RNA Polymerase II Variant, and Regulates Metabolic Genes in ESCs
  Dynamic long-range chromatin interactions control Myb proto-oncogene transcription during erythroid development
  CENP-C facilitates the recruitment of M18BP1 to centromeric chromatin.
  Connecting Threads: Epigenetics and Metabolism
  Roles of long, non-coding RNA in chromosome-wide transcription regulation: Lessons from two dosage compensation systems
  Cytosolic DNA Triggers Mitochondrial Apoptosis via DNA Damage Signaling Proteins Independently of AIM2 and RNA Polymerase III

2011

  Histidine kinases and response regulators in networks
  Probing the conformation of the ISWI ATPase domain with genetically encoded photoreactive crosslinkers and mass spectrometry
  Engineering antibodies and proteins for molecular in vivo imaging
  In breast cancer, a high ratio of tumour-infiltrating intraepithelial CD8+ to FoxP3+ cells is characteristic for the medullary subtype
  CD103 is a hallmark of tumor-infiltrating regulatory T cells
  Developmental regulation of N-terminal H2B methylation in Drosophila melanogaster
  De novo assembly of a PML nuclear subcompartment occurs through multiple pathways and induces telomere elongation
  The MOF-containing NSL complex associates globally with housekeeping genes, but activates only a defined subset
  Cdc14 phosphatase promotes segregation of telomeres through repression of RNA polymerase II transcription
  Suv4-20h Abrogation Enhances Telomere Elongation during Reprogramming and Confers a Higher Tumorigenic Potential to iPS Cells
  Regulation of DNA methyltransferase 1 by interactions and modifcations
  Proteomic Interrogation of Human Chromatin
  Histone hypoacetylation is required to maintain late replication timing of constitutive heterochromatin
  Estrogen-Dependent Gene Transcription in Human Breast Cancer Cells Relies upon Proteasome-Dependent Monoubiquitination of Histone H2B
  Cooperative DNA and Histone Binding by Uhrf2 Links the Two Major Repressive Epigenetic Pathways
  Splicing enhances recruitment of methyltransferase HYPB/Setd2 and methylation of histone H3 Lys36
  Stage-Specific Histone Modification Profiles Reveal Global Transitions in the Xenopus Embryonic Epigenome
  Wogonin and related natural flavones are inhibitors of CDK9 that induce apoptosis in cancer cells by transcriptional suppression of Mcl-1
  Tuning communication fidelity
  Transcription initiation platforms and GTF recruitment at tissue-specific enhancers and promoters
  Role for hACF1 in the G2/M damage checkpoint
  Recognition of 5-Hydroxymethylcytosine by the Uhrf1 SRA Domain
  Systemic Cancer Therapy with a Small Molecule Agonist of Toll-like Receptor 7 Can Be Improved by Circumventing TLR Tolerance
  The RNA Helicase Rm62 Cooperates with SU(VAR)3-9 to Re-Silence Active Transcription in Drosophila melanogaster
  Twists and turns of DNA methylation
  Kirsten Jung's program of emphasis on individuality of bacteria gets DFG funding
  Detection and function of an intramolecular disulfide bond in the pH-responsive CadC of Escherichia coli
  Functional Nuclear Organization of Transcription and DNA Replication
  The C-Terminal Domain of RNA Polymerase II Is Modified by Site-Specific Methylation
  H3 Lysine 4 Is Acetylated at Active Gene Promoters and Is Regulated by H3 Lysine 4 Methylation
  Sequential Establishment of Marks on Soluble Histones H3 and H4
  Identification of ArgP and Lrp as Transcriptional Regulators of lysP, the Gene Encoding the Specific Lysine Permease of Escherichia coli
  PARG is recruited to DNA damage sites through poly(ADP-ribose)- and PCNA-dependent mechanisms
  Global Analysis of the Relationship between JIL-1 Kinase and Transcription
  Kirsten Jung Interview about CIPSMs Gender Equality Program
  Characterization of PvuRts1I endonuclease as a tool to investigate genomic 5–hydroxymethylcytosine
  Histone acetylation controls the inactive X chromosome replication dynamics
  Transcription modulation chromosome-wide: universal features and principles of dosage compensation in worms and flies
  H4K20 monomethylation faces the WNT
  Cortical Constriction During Abscission Involves Helices of ESCRT-III–Dependent Filaments
  Interferon Antagonist NSs of La Crosse Virus Triggers a DNA Damage Response-like Degradation of Transcribing RNA Polymerase II
  Different Binding Properties and Function of CXXC Zinc Finger Domains in Dnmt1 and Tet1
  Usp7 and Uhrf1 control ubiquitination and stability of the maintenance DNA methyltransferase Dnmt1
  Crystal structure of the sensory domain of Escherichia coli CadC, a member of the ToxR-like protein family
  Nuclei of chicken neurons in tissues and three-dimensional cell cultures are organized into distinct radial zones
  CpG Blocks Immunosuppression by Myeloid-Derived Suppressor Cells in Tumor-Bearing Mice
  New Insights into the Signaling Mechanism of the pH-responsive, Membrane-integrated Transcriptional Activator CadC of Escherichia coli

2010

  The histone methyltransferase Dot1 is required for DNA damage repair and proper development in Dictyostelium
  Delivery of Immunostimulatory RNA Oligonucleotides by Gelatin Nanoparticles Triggers an Efficient Antitumoral Response
  Every methyl counts – Epigenetic calculus
  Fast signals and slow marks: the dynamics of histone modifications
  CIPSM sponsors Kirsten Jung's project at the International Genetically Engineered Machine competition (iGEM)
  Nucleosome dynamics and epigenetic stability
  Developmental role for ACF1-containing nucleosome remodellers in chromatin organisation
  Identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y
  Chemical-genomic dissection of the CTD code
  Dosage compensation and the global re-balancing of aneuploid genomes
  Efficient Eradication of Subcutaneous but Not of Autochthonous Gastric Tumors by Adoptive T Cell Transfer in an SV40 T Antigen Mouse Model
  Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA
  Form and function of dosage-compensated chromosomes – a chicken-and-egg relationship
  A guide to super-resolution fluorescence microscopy
  Soft skills turned into hard facts: nucleosome remodelling at developmental switches
  The Activation Potential of MOF Is Constrained for Dosage Compensation
  Remodeling of nuclear architecture by the thiodioxoxpiperazine metabolite chaetocin
  Epigenetic regulation of development by histone lysine methylation
  CIPSM Excursion to Bayer Schering Pharma with Kirsten Jung
  The tumor suppressor p53 connects ribosome biogenesis to cell cycle control: a double-edged sword
  Novel antibody derivatives for proteome and high-content analysis
  Phosphorylation of SU(VAR)3–9 by the Chromosomal Kinase JIL-1
  Heterochromatin dysregulation in human diseases
  Top-Down de Novo Protein Sequencing of a 13.6 kDa Camelid Single Heavy Chain Antibody by Matrix-Associated Laser Desorption Ionization-Time-of-Flight/Time-of-Flight Mass Spectrometry
  Phosphorylation of histone H3T6 by PKCbI controls demethylation at histone H3K4
  Replication Stress Interferes with Histone Recycling and Predeposition Marking of New Histones
  Chemotherapeutic drugs inhibit ribosome biogenesis at various levels
  The complexityofthe‘simple’two-component systemKdpD/KdpE in Escherichia coli
  Activation of Melanoma Differentiation-Associated Gene 5 Causes Rapid Involution of the Thymus
  Immunostimulatory RNA Blocks Suppression by Regulatory T Cells
  The DNA binding CXC domain of MSL2 is required for faithful targeting the Dosage Compensation Complex to the X chromosome
  Defects in 18S or 28S rRNA processing activate the p53 pathway
  RNA Polymerase II C-terminal Heptarepeat Domain Ser-7 Phosphorylation Is Established in a Mediator-dependent Fashion

2009

  The multi-domain protein Np95 connects DNA methylation and histone modification
  Modulation of protein properties in living cells using nanobodies
  Interaction of HP1 and Brg1/Brm with the Globular Domain of Histone H3 Is Required for HP1-Mediated Repression
  Superior Protective Immunity against Murine Listeriosis by Combined Vaccination with CpG DNA and Recombinant Salmonella enterica Serovar Typhimurium
  Immunostimulatory RNA Oligonucleotides Induce an Effective Antitumoral NK Cell Response through the TLR71
  Recognition of RNA virus by RIG-I results in activation of CARD9 and inflammasome signaling for interleukin 1beta production
  A New Mechanism of Phosphoregulation in Signal Transduction Pathways
  RNAPII CTD SER-7 phosphorylation is established in a mediator-dependent fashion
  Measurement of replication structures at the nanometer scale using super-resolution light microscopy
  CIPSM researcher Prof. Dr. Thomas Cremer is honored by the German Academy of Sciences Leopoldina with the Schleiden Medal
  Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells
  Bundesministerium supports CIPSM cooperation of CIPSM Investigators Leonhardt and Schotta
  Revealing the high-resolution three-dimensional network of chromatin and interchromatin space: A novel electron-microscopic approach to reconstructing nuclear architecture
  CIPSM football team rules the cup!
  The regulatory interplay between membrane-integrated sensors and transport proteins in bacteria
  The dosage compensation complex shapes the conformation of the X chromosome in Drosophila
  A Technical Note on Quantum Dots for Multi-Color Fluorescence in situ Hybridization
  Induction Kinetics of a Conditional pH Stress Response System in Escherichia coli
  Proteome-wide prediction of acetylation substrates
  Proapoptotic signaling induced by RIG-I and MDA-5 results in type I interferon–independent apoptosis in human melanoma cells
  Heterogeneity in quorum sensing-regulated bioluminescence of Vibrio harveyi
  Changes of higher order chromatin arrangements during major genome activation in bovine preimplantation embryos
  Domain swapping reveals that the N-terminal domain of the sensor kinase KdpD in Escherichia coli is important for signaling
  CDK9 directs H2B monoubiquitination and controls replication-dependent histone mRNA 3'-end processing
  5'-triphosphate RNA requires base-paired structures to activate antiviral signaling via RIG-I
  DNA Methylation-Mediated Epigenetic Control
  Chromosome shattering: a mitotic catastrophe due to chromosome condensation failure
  Establishment of Histone Modifications after Chromatin Assembly
  MBD4 and MLH1 are required for apoptotic induction in xDNMT1-depleted embryos
  The HP1a–CAF1–SetDB1-containing complex provides H3K9me1 for Suv39-mediated K9me3 in pericentric heterochromatin
  TFIIH Kinase Places Bivalent Marks on the Carboxy-Terminal Domain of RNA Polymerase II
  Stimulation of the potassium sensor KdpD kinase activity by interaction with the phosphotransferase protein IIANtr in Escherichia coli
  Drosophila ISWI Regulates the Association of Histone H1 With Interphase Chromosomes in Vivo
  Nuclear Architecture of Rod Photoreceptor Cells Adapts to Vision in Mammalian Evolution
  Drosophila HP1c Is Regulated by an Auto-Regulatory Feedback Loop through Its Binding Partner Woc
  Synergy between CD26/DPP-IV Inhibition and G-CSF Improves Cardiac Function after Acute Myocardial Infarction
  Active promoters and insulators are marked by the centrosomal protein 190
  How are signals transduced across the cytoplasmic membrane? Transport proteins as transmitter of information
  Spatiotemporal Dynamics of Regulatory Protein Recruitment at DNA Damage Sites
  Dimerization of DNA Methyltransferase 1 Is Mediated by Its Regulatory Domain
  Backbone Structure of Transmembrane Domain IX of the Naþ/Proline Transporter PutP of Escherichia coli
  A versatile non-radioactive assay for DNA methyltransferase activity and DNA binding
  Monomethylation of Lysine 20 on Histone H4 Facilitates Chromatin Maturation

2008

  The Chromosomal High-Affinity Binding Sites for the Drosophila Dosage Compensation Complex
  The Universal Stress Protein UspC Scaffolds the KdpD/KdpE Signaling Cascade of Escherichia coli under Salt Stress
  Recruitment of RNA polymerase II cofactor PC4
  5´-triphosphate-siRNA: turning gene silencing and Rig-I activation against melanoma
  Short-term activation induces multifunctional dendritic cells that generate potent antitumor T-cell responses in vivo
  Discontinuous movement of mRNP particles in nucleoplasmic regions devoid of chromatin
  Identifying specific protein interaction partners using quantitative mass spectrometry and bead proteomes
  Camelid immunoglobulins and nanobody technology
  Combined Use of RNAi and Quantitative Proteomics to Study Gene Function in Drosophila
  PR-SET7 and SUV4-20H regulate H4 lysine-20 methylation at imprinting control regions in the mouse
  Function of Transmembrane Domain IX in the Na+/Proline Transporter PutP
  A fluorescent two-hybrid (F2H) assay for direct visualization of protein interactions in living cells
  Uncoupling the replication machinery
  Targeting CpG Oligonucleotides to the Lymph Node by Nanoparticles Elicits Efficient Antitumoral Immunity
  Nuclear transit of the RNA-binding protein She2 is required for translational control of localized ASH1 mRNA
  Subdiffraction Multicolor Imaging of the Nuclear Periphery with 3D Structured Illumination Microscopy
  A chromatin-wide transition to H4K20 monomethylation impairs genome integrity and programmed DNA rearrangements in the mouse
  The PHD Domain of Np95 (mUHRF1) Is Involved in Large-Scale Reorganization of Pericentromeric Heterochromatin
  Photorhabdus luminescens genes induced upon insect infection
  Simultaneous Transport of Different Localized mRNA Species Revealed by Live-Cell Imaging
  Molecular evolution of the RNA polymerase II CTD
  Generation and Characterization of a Rat Monoclonal Antibody Specific for Multiple Red Fluorescent Proteins
  Simple generation of site-directed point mutations in the Escherichia coli chromosome using Red®/ET® Recombination
  DNA vaccination efficiently induces antibodies to Nogo-A and does not exacerbate experimental autoimmune encephalomyelitis
  ATP-dependent chromatosome remodeling
  CIPSM-Researcher Heinrich Leonhardt wins Binder-Innovation Award 2008
  DNA sequence and the organization of chromosomal domains
  The nucleolar SUMO-specific protease SENP3 reverses SUMO modification of nucleophosmin and is required for rRNA processing
  Fine Mapping of Posttranslational Modifications of the Linker Histone H1 from Drosophila melanogaster
  Chromatin proteomics and epigenetic regulatory circuits

2007

  Structure-function analysis of the RNA helicase maleless
  Seven Ups the Code
  Transcribing RNA Polymerase II Is Phosphorylated at CTD Residue Serine-7
  Serine-7 of the RNA Polymerase II CTD Is Specifically Required for snRNA Gene Expression
  Structure–function analysis of the RNA polymerase cleft loops elucidates initial transcription, DNA unwinding and RNA displacement
  Probing Intranuclear Environments at the Single-Molecule Level
  NC2 mobilizes TBP on core promoter TATA boxes
  A mutagenesis strategy combining systematic alanine scanning with larger mutations to study protein interactions
  Feedback-regulated poly(ADP-ribosyl)ation by PARP-1 is required for rapid response to DNA damage in living cells
  A Versatile Nanotrap for Biochemical and Functional Studies with Fluorescent Fusion Proteins
  Maintenance of imprinting and nuclear architecture in cycling cells




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TU München
MPG
Helmholz Muenchen
MPI of Neurobiology
MPI of Biochemistry