Universal sample preparation method for proteome analysis

Nature Methods, 2009, 6, 359 - 62 published on 19.04.2009
Nature Methods, online article
We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry–based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.

mann_nature_methods_09_500

 




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