Near-infrared branding efficiently correlates light and electron microscopy

Nature Methods, 2011, doi:10.1038/nmeth.1622, 568–570 (2011) published on 05.06.2011
Nature Methods, online article
The correlation of light and electron microscopy of complex tissues remains a major challenge. Here we report near-infrared branding (NIRB), which facilitates such correlation by using a pulsed, near-infrared laser to create defined fiducial marks in three dimensions in fixed tissue. As these marks are fluorescent and can be photo-oxidized to generate electron contrast, they can guide re-identification of previously imaged structures as small as dendritic spines by electron microscopy.

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