Rapid Matrix-Assisted Refolding of Histidine-Tagged Proteins

ChemBioChem, 2009, 10(5), 869 - 876, doi:10.1002/cbic.200800697 published on 23.02.2009
ChemBioChem, online article
Matrix refolded: The formation of inclusion bodies, which are amorphous aggregates of misfolded insoluble protein, during recombinant protein expression, is one of the biggest bottlenecks in protein science. We report a stepwise, rational optimization procedure for refolding of insoluble proteins (see scheme). In comparison to refolding in-solution, this parallelized, matrix-assisted approach allows the refolding of various proteins in a fast and efficient manner.

TU München
Helmholtz München
MPI of Neurobiology
MPI of Biochemistry