Structure and Reaction Mechanism of Pyrrolysine Synthase (PylD)
01-Jul-2013
Angewandte Chemie, 2013, DOI: 10.1002/anie.201301164, Volume 52, Issue 27, pages 7033–7037, published on 01.07.2013
The final step in the biosynthesis of the 22nd genetically encoded amino acid, pyrrolysine, is catalyzed by PylD, a structurally and mechanistically unique dehydrogenase. This catalyzed reaction includes an induced-fit mechanism achieved by major structural rearrangements of the N-terminal helix upon substrate binding. Different steps of the reaction trajectory are visualized by complex structures of PylD with substrate and product.