The radical SAM enzyme spore photoproduct lyase employs a tyrosyl radical for DNA repair
Chemical Communications, 2012, DOI: 10.1039/C2CC37735G, 49, 722-724 published on 28.11.2012
The spore photoproduct lyase is a radical SAM enzyme, which repairs 5-(α-thyminyl)-5,6-dihydrothymidine. Here we show that the enzyme establishes a complex radical transfer cascade and creates a cysteine and a tyrosyl radical dyade to establish repair. This allows the enzyme to solve topological and energetic problems associated with the radical based repair reaction.