Research Area E - Publications 2017

Nature Chemical Biology, volume 14, pages 72–78, doi:10.1038/nchembio.2531
Nature Chemical Biology, online article


Tet enzymes oxidize 5-methyl-deoxycytidine (mdC) to 5-hydroxymethyl-dC (hmdC), 5-formyl-dC (fdC) and 5-carboxy-dC (cadC) in DNA. It was proposed that fdC and cadC deformylate and decarboxylate, respectively, to dC over the course of an active demethylation process. This would re-install canonical dC bases at previously methylated sites. However, whether such ...


Angew. Chem. Int. Ed., Volume 56, Issue 46, Pages 14536–14539, DOI: 10.1002/anie.201708178
Angew. Chem. Int. Ed., online article


Hemithioindigo molecular motors undergo very fast unidirectional rotation upon irradiation with visible light, which has prevented a complete analysis of their working mechanism. In this work, we have considerably slowed down their motion by using a new synthesis for sterically hindered motor derivatives. This method allowed the first observation of all four ...


mBio, vol. 8, no. 5 e01412-17, doi: 10.1128/mBio.01412-17
mBio, online article


Glycosylation is a universal strategy to posttranslationally modify proteins. The recently discovered arginine rhamnosylation activates the polyproline-specific bacterial translation elongation factor EF-P. EF-P is rhamnosylated on arginine 32 by the glycosyltransferase EarP. However, the enzymatic mechanism remains elusive. In the present study, we solved the ...


J. Am. Chem. Soc., 139 (42), pp 15060–15067, DOI: 10.1021/jacs.7b07531
J. Am. Chem. Soc., online article


Hemiindigo is a long known chromophore that absorbs in the blue part of the spectrum but has almost completely been ignored as potential photoswitch. Herein we show how the absorption of hemiindigo is shifted to the red part of the visible spectrum and how nearly perfect photoswitching can be achieved using blue or green and red light. Five derivatives were ...


ChemBioChem, DOI: 10.1002/cbic.201700209
ChemBioChem, oline article


The in vivo incorporation of alkyne-modified bases into the genome of cells is today the basis for the efficient detection of cell proliferation. Cells are grown in the presence of ethinyl-dU (EdU), fixed and permeabilised. The incorporated alkynes are then efficiently detected by using azide-containing fluorophores and the CuI-catalysed alkyne–azide click ...


J. Am. Chem. Soc., 139 (30), pp 10359–10364, DOI: 10.1021/jacs.7b04131
J. Am. Chem. Soc., online article


5-Formyl-dC (fdC) and 5-carboxy-dC (cadC) are newly discovered bases in the mammalian genome that are supposed to be substrates for base excision repair (BER) in the framework of active demethylation. The bases are recognized by the monofunctional thymine DNA glycosylase (Tdg), which cleaves the glycosidic bond of the bases to give potentially harmful abasic ...


2015, published on 31.08.2015


Das Institut für chemische Epigenetik (ICEM) mit einer Nutzfläche von 3.430 m² und einer Fördersumme von 38,73 Millionen Euro hat eine Förderempfehlung von Seiten des Wissenschaftsrates erhalten.
Das ICEM basiert auf dem CIPSM und wird den Forschungsschwerpunkt der chemischen Epigenetik erforschen.

Der Forschungsbau wird auf dem HighTech Campus ...


The EMBO Journal, DOI 10.15252/embj.201695757
The EMBO Journal, online article


Replacement of canonical histones with specialized histone variants promotes altering of chromatin structure and function. The essential histone variant H2A.Z affects various DNA-based processes via poorly understood mechanisms. Here, we determine the comprehensive interactome of H2A.Z and identify PWWP2A as a novel H2A.Z-nucleosome binder. PWWP2A is a ...


ChemBioChem, Volume 18, Issue 14, Pages 1379–1382, DOI: 10.1002/cbic.201700169
ChemBioChem, online article


Aromatic amines are strongly carcinogenic. They are activated in the liver to give reactive nitrenium ions that react with nucleobases within the DNA duplex. The reaction occurs predominantly at the C8 position of the dG base, thereby giving C8-acetyl-aryl- or C8-aryl-dG adducts in an electrophilic aromatic substitution reaction. Alternatively, reaction with the ...


Angew. Chem. Int. Ed., Volume 56, Issue 37, Pages 11268–11271, DOI: 10.1002/anie.201700424
Angew. Chem. Int. Ed., online article


Until recently, it was believed that the genomes of higher organisms contain, in addition to the four canonical DNA bases, only 5-methyl-dC (m5dC) as a modified base to control epigenetic processes. In recent years, this view has changed dramatically with the discovery of 5-hydroxymethyl-dC (hmdC), 5-formyl-dC (fdC), and 5-carboxy-dC (cadC) in DNA from stem cells ...


Chem. Eur. J., Volume 23, Issue 26, Pages 6237–6243, DOI: 10.1002/chem.201700826
Chem. Eur. J., online article


Photoswitches reacting to visible light instead of harmful UV irradiation are of very high interest due to the mild and broadly compatible conditions of their operation. Shifting the absorption into the red region of the electromagnetic spectrum usually comes at the cost of losing thermal stability of the metastable state—the switch switches off by itself. Only ...


J. Phys. Chem. Lett., 2017, 8 (7), pp 1585–1592, DOI: 10.1021/acs.jpclett.7b00371
J. Phys. Chem. Lett., online article


Twisted intramolecular charge transfer (TICT) formation in hemithioindigo photoswitches has recently been reported and constitutes a second deexcitation pathway complementary to photoisomerization. Typically, this behavior is not found for this type of photoswitches, and it takes special geometric and electronic conditions to realize it. Here we present a ...


Angew. Chem. Int. Ed. 2017, 56, 1 – 6, DOI: 10.1002/anie.201611063
Angew. Chem. Int. Ed., online article


Nature provides an inexhaustible diversity of small organic molecules with beautiful molecular architectures that have strong and selective inhibitory activities. However, this tremendous biomedical potential often remains inaccessible, as the structural complexity of natural products can render their synthetic preparation extremely challenging. This problem is ...


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