Research Area A - Publications 2020
Recent evolution of a TET-controlled and DPPA3/STELLA-driven pathway of passive DNA demethylation in mammals
Nat Commun 11, 5972 (2020), https://doi.org/10.1038/s41467-020-19603-1
Nat Commun., online article
Genome-wide DNA demethylation is a unique feature of mammalian development and naïve pluripotent stem cells. Here, we describe a recently evolved pathway in which global hypomethylation is achieved by the coupling of active and passive demethylation. TET activity is required, albeit indirectly, for global demethylation, which mostly occurs at sites devoid of TET ...
Nat Commun 11, 4321 (2020). https://doi.org/10.1038/s41467-020-18063-x
Nat.Commun., online article
Bacterial colonization of the human intestine requires firm adhesion of bacteria to insoluble substrates under hydrodynamic flow. Here we report the molecular mechanism behind an ultrastable protein complex responsible for resisting shear forces and adhering bacteria to cellulose fibers in the human gut. Using single-molecule force spectroscopy (SMFS), ...
J. Phys. Chem. Lett. 2020, 11, 11, 4462–4480, https://doi.org/10.1021/acs.jpclett.9b03833
J. Phys. Chem. Lett., online article
Self-healing dyes have emerged as a new promising class of fluorescent labels. They consist of two units, a fluorescent dye and a photostabilizer. The latter heals whenever the fluorescent dye is in danger of taking a reaction pathway toward photobleaching. We describe the underlying concepts and summarize the developmental history and state-of-the-art, including ...
DNA origami-based single-molecule force spectroscopy elucidates RNA Polymerase III pre-initiation complex stability
Nat Commun 11, 2828 (2020), https://doi.org/10.1038/s41467-020-16702-x
Nat Commun, online article
The TATA-binding protein (TBP) and a transcription factor (TF) IIB-like factor are important constituents of all eukaryotic initiation complexes. The reason for the emergence and strict requirement of the additional initiation factor Bdp1 in the RNA polymerase (RNAP) III system, however, remained elusive. A poorly studied aspect in this context is the effect of ...