Research Area E - Publications 2020
Recent evolution of a TET-controlled and DPPA3/STELLA-driven pathway of passive DNA demethylation in mammals
Nat Commun 11, 5972 (2020), https://doi.org/10.1038/s41467-020-19603-1
Nat Commun., online article
Genome-wide DNA demethylation is a unique feature of mammalian development and naïve pluripotent stem cells. Here, we describe a recently evolved pathway in which global hypomethylation is achieved by the coupling of active and passive demethylation. TET activity is required, albeit indirectly, for global demethylation, which mostly occurs at sites devoid of TET ...
Systematic analysis of the binding behaviourof UHRF1 towards different methyl- and carboxylcytosine modification patterns at CpG dyads
PLoS ONE, 15(2): e0229144, https://doi.org/10.1371/journal.pone.0229144
PLoS ONE, online article
The multi-domain protein UHRF1 is essential for DNA methylation maintenance and binds DNA via a base-flipping mechanism with a preference for hemi-methylated CpG sites. We investigated its binding to hemi- and symmetrically modified DNA containing either 5-methylcytosine (mC), 5-hydroxymethylcytosine (hmC), 5-formylcytosine (fC), or 5-carboxylcytosine (caC). Our ...