Research Area D - Publications 2020

Cell Rep., [online ahead of print] , DOI:https://doi.org/10.1016/j.celrep.2020.107522
Cell Rep., online article

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Tumor cells orchestrate their microenvironment. Here, we provide biochemical, structural, functional, and clinical evidence that Cathepsin S (CTSS) alterations induce a tumor-promoting immune microenvironment in follicular lymphoma (FL). We found CTSS mutations at Y132 in 6% of FL (19/305). Another 13% (37/286) had CTSS amplification, which was associated with ...

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Nature Communications, volume 11, Article number: 1222 (2020), https://doi.org/10.1038/s41467-020-15006-4
Nature Communications, online article

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Stable inheritance of DNA methylation is critical for maintaining differentiated phenotypes in multicellular organisms. We have recently identified dual mono-ubiquitylation of histone H3 (H3Ub2) by UHRF1 as an essential mechanism to recruit DNMT1 to chromatin. Here, we show that PCNA-associated factor 15 (PAF15) undergoes UHRF1-dependent dual mono-ubiquitylation ...

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PLoS ONE, 15(2): e0229144, https://doi.org/10.1371/journal.pone.0229144
PLoS ONE, online article

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The multi-domain protein UHRF1 is essential for DNA methylation maintenance and binds DNA via a base-flipping mechanism with a preference for hemi-methylated CpG sites. We investigated its binding to hemi- and symmetrically modified DNA containing either 5-methylcytosine (mC), 5-hydroxymethylcytosine (hmC), 5-formylcytosine (fC), or 5-carboxylcytosine (caC). Our ...

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Nat Commun. 11, 304 (2020) doi:10.1038/s41467-019-14160-8
Nat Commun., online article

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Biological processes in development and disease are controlled by the abundance, localization and modification of cellular proteins. We have developed versatile tools based on recombinant E3 ubiquitin ligases that are controlled by light or drug induced heterodimerization for nanobody or DARPin targeted depletion of endogenous proteins in cells and organisms. We ...

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