Research Area D - Publications 2019

Leukemia, https://doi.org/10.1038/s41375-019-0497-6
Leukemia, online article

leonhardt-schotta-2019_stief_leukemia_550.100x0.jpg

Acute myeloid leukemia (AML) is an aggressive hematologic neoplasm resulting from the malignant transformation of myeloid progenitors. Despite intensive chemotherapy leading to initial treatment responses, relapse caused by intrinsic or acquired drug resistance represents a major challenge. Here, we report that histone 3 lysine 27 demethylase KDM6A (UTX) is ...

|READ MORE|

Nucleic Acids Research, 2019, 1, doi: 10.1093/nar/gkz466
Nucleic Acids Research, online article

leonhardt_2019_tepper_nar_550.100x0.jpg

Affinity maturation of the humoral immune response depends on somatic hypermutation (SHM) of immunoglobulin (Ig) genes, which is initiated by targeted lesion introduction by activation-induced deaminase (AID), followed by error-prone DNA repair. Stringent regulation of this process is essential to prevent genetic instability, but no negative feedback control has ...

|READ MORE|

Org. Biomol. Chem., 2019, DOI: 10.1039/c9ob00508k
Org. Biomol. Chem., online article

leonhardt-2019_stengl_orgbiomolchem_550.100x0.jpeg

We introduce a chemoenzymatic strategy for straightforward in vitro generation of C-terminally linked fusion proteins. Tubulin tyrosine ligase is used for the incorporation of complementary click chemistry handles facilitating subsequent formation of functional bispecific antibody-fragments. This simple strategy may serve as central conjugation hub for a modular ...

|READ MORE|

Angewandte Chemie International Edition, https://doi.org/10.1002/anie.201814715
Angewandte Chemie International Edition, online article

leonhardt-2019_kasper_angewchemieint_550.100x0.jpeg

We describe a new technique in protein synthesis that extends the existing repertoire of methods for protein modification: A chemoselective reaction that induces reactivity for a subsequent bioconjugation. Hereby, an azide building block reacts first with an ethynylphosphonite via the Staudinger‐phosphonite reaction (SPhR) to an ethynylphosphonamidate. The ...

|READ MORE|

Journal of Bacteriology, Volume 201, Issue 3, e00610-18, https://doi.org/10.1128/JB.00610-18
Journal of Bacteriology, online article

jung-vilhena_et_al__2019_550.100x0.jpeg

Escherichia coli and many other bacterial species can enter into a viable but nonculturable (VBNC) state, which is a survival strategy adopted by cells exposed to adverse environmental conditions. Pyruvate is known to be one factor that promotes resuscitation of VBNC cells. Here we studied the role of a pyruvate-sensing network, composed of the histidine ...

|READ MORE|

Nucleic Acids Research, 2019, 1, doi: 10.1093/nar/gky1320
Nucleic Acids Research, online article

papenfort-nwg-d-gky1320_550.100x0.jpeg

Bacteria use quorum sensing to monitor cell density and coordinate group behaviours. In Vibrio cholerae, the causative agent of the diarrheal disease cholera, quorum sensing is connected to virulence gene expression via the two autoinducer molecules, AI-2 and CAI-1. Both autoinducers share one signal transduction pathway to control the production of AphA, a key ...

|READ MORE|

COMMUNICATIONS BIOLOGY, (2019) 2:4, https://doi.org/10.1038/s42003-018-0248-7
COMMUNICATIONS BIOLOGY, online article

jung-brameyer_et_al_550.100x0.jpeg

All living cells have a large number of proteins that are anchored with one transmembrane helix in the cytoplasmic membrane. Almost nothing is known about their spatiotemporal organization in whole cells. Here we report on the localization and dynamics of one representative, the pH sensor and transcriptional regulator CadC in Escherichia coli. Fluorophore-tagged ...

|READ MORE|

CIPSM Movie
LMUexcellent
TU München
MPG
Helmholtz München
MPI of Neurobiology
MPI of Biochemistry