Novel antibody derivatives for proteome and high-content analysis
Analytical and Bioanalytical Chemistry, 2010, doi: 10.1007/s00216-010-3657-0, 397(8): 3203–3208. published on 07.04.2010
Analytical and Bioanalytical Chemistry, online article
The understanding of cellular processes and their pathophysiological alterations requires comprehensive data on the abundance, distribution, modification, and interaction of all cellular components. On the one hand, artificially introduced fluorescent fusion proteins provide information about their distribution and dynamics in living cells but not about endogenous factors. On the other hand, antibodies can detect endogenous proteins, posttranslational modifications, and other cellular components but mostly in fixed and permeabilized cells. Here we highlight a new technology based on the antigen-binding domain of heavy-chain antibodies (VHH) from Camelidae. These extremely stable VHH domains can be produced in bacteria, coupled to matrices, and used for affinity purification and proteome.